QIAcard FTA® products from GE Healthcare Whatman® provide users with an established range of effective tools and innovative solutions for the collection, purification and transportation of nucleic acids.
FTA® is a chemical treatment that allows for the rapid isolation of pure DNA. When samples are applied to FTA®-treated paper or card, cell lysis occurs and high molecular weight DNA is immobilised within the matrix. Amplification or restriction enzyme digestion can be performed directly on the treated paper without the need for extensive extraction procedures.
- Rapid isolation of pure DNA
- Designed to kill pathogens and prevent future colonisation by bacteria of fungi
- Protects DNA from microbial and environmental degradation
- Archive samples at room temperature
- Reduces potential for cross-contamination between samples
- Eliminates shearing forces associated with conventional extraction methods
- Capture and store nucleic acids in one step
- Reduces handling, saving time and money
- Stabilises genomic DNA at room temperature for over eleven years
QIAcard FTA CloneSaver™ cards capture BAC and Plasmid DNA for long term storage and downstream applications in one easy step.
- Simply apply 5µl bacterial culture, resuspended colony or glycerol stock to a CloneSaver™ card. The plasmid or BAC DNA is captured and stabilised for long-term storage or immediate processing
- Captured DNA is easily accessible for downstream applications
- Store up to 96 samples on each CloneSaver™ card
- CloneSaver™ cards include a pink indicator that turns white upon sample application to aid identification and location
- Plasmid DNA held on CloneSaver™ cards are stable at room temperature for at least two and a half years
CloneSaver™ cards provide a convenient and accessible format for down-stream applications involving frequently used, plasmid and BAC clones. Washed, punched discs from the card are ready for transformation of bacteria by stored, immobilised, plasmid DNA using electroporation or heat-shock methods. An immobilised, plasmid DNA punch can also be used directly as a template for PCR. The plasmid DNA template remains bound to the punch and can be re-used in another reaction. The PCR products remain in solution, do not bind to the punch, and are easily recoverable.